Dados do Trabalho

Título

Signature profile of immune mediators in patients with recent and late lesions of localized cutaneous leishmaniasis.

Introdução

The immune response is essential in combating diseases, and the profile of the response can vary according to the need. In localized cutaneous leishmaniasis (LCL), there is initially a predominance of type I response, which is essential for parasite elimination in cutaneous lesions, and subsequently, the response shifts to type II to contain tissue damage and initiate tissue repair. It is known that the time of infection influences the profile of the immune response.

Objetivo (s)

Therefore, this study evaluated the impact of the time of evolution of the cutaneous lesion on the immune response profile. 

Material e Métodos

Thus, patients with cutaneous lesions who had positive parasitological or molecular diagnosis for leishmaniasis were included and grouped in recent lesion (up to 2 months of evolution) and late lesion (more than 2 months of evolution). In this study, 15 patients with recent lesions (RL) and 20 patients with late lesions (LL) were included. Chemokines, cytokines, and growth factors levels were quantified in peripheral blood samples and biopsy fragments using the Bio-Plex Pro Human Cytokine 27 kit. The study was approved by the Ethics Committee of the René Rachou Institute (number: 2,401,147-2017).

Resultados e Conclusão

The results showed that the time of lesion evolution had a significant impact on the evaluated immune mediators, allowing the segregation of a distinct immune profile among its subgroups. Signature analysis demonstrated that the LL group presented a greater number of circulating biomarkers (CXCL8, CCL11, CCL3, CCL4, CCL2, CCL5, CXCL10, IL-1β, IL-6, TNF-a, IFN-g, IL-15, IL-17, IL-1Ra, IL-4, IL-5, IL-9, IL-10, FGF, PDGF, VEGF, G-CSF, GM-CSF, IL-7, and IL-2). On the other hand, in the RL group was observed increase of immune mediators in the inflammatory exudate (CXCL8, CCL11, CCL2, CCL5, IL-1b, IL-6, TNF-a, IL-12, IFN-g, IL-15, IL-17, IL-1Ra, IL-4, IL-5, IL-9, IL-13, PDGF, VEGF, G-CSF, GM-CSF, and IL-2). Correlation analyses showed a slight increase in the connections, mainly of pro-inflammatory cytokines and growth factors axes in the RL group, while in the LL group, was observed a balance of connections. The results obtained in this study showed that patients with recent lesions and patients with late lesions presented distinct immune response profile. This approach can provide important information about development vs healing of cutaneous lesions.

Palavras Chave

Localized cutaneous leishmaniasis; time of lesion; immunological biomarkers