Dados do Trabalho

Título

Identification of the Insect-Specific virus circulation in mosquitoes collected from a dengue hyperendemic city

Introdução

The Insect-Specific vírus (ISV) are a virus group that it able to infect only insect’s cells. The first ISV identification occurred more than 40 years. Since then, with the advancement of molecular biology and bioinformatics the new ISV are discovered all the time; many studies are conducted about the ISV importance about the arbovirus transmission. To discover which mechanisms are involved in a possible coinfection between ISV and arbovirus and its persistence in the nature during the disadvantage period at transmission are needed, principally in an endemics areas such Brazil

Objetivo (s)

We proposed to realize an entomological survey the major´s mosquitoes SJRP native, and to investigate which ISV circling naturally in these species

Material e Métodos

We realized the mosquito’s collection from October 2023 until April 2024; we always installed the traps in the second fortnight each month. To collect the adults mosquitoes we using the BG-Sentinel traps. We tested the samples to most abundant ISV in Aedes mosquitoes - Humaiatá-Tubiacanga vírus (HTV) (no family classification) and Phasi Charoen-like vírus (PCLV) (Family: Phenuviridae). This two ISV were chosen because some studies show its influence about the arbovirus transmission in the nature. All positive sample were sequencing using the kit BigDye Terminator Cycle Sequencing. The software Geneious prime were used to build a consensus sequence and the virus identification were conducted with the Blastn.

Resultados e Conclusão

We found the 80,6% at the samples were PCLV and/or HTV positive. The PCLV occurred in 38.5% at the samples, HTV occurred in 4.4% at the samples, and we can observed a total 37.7% at the coinfection PCLV/HTV samples. The Jaguaré-João Paulo II (88,9%) neighborhood were the most positive the ISV occurrence, follow  Parque Industrial (86,7%), Solo Sagrado (86%), São Deocleciano (85%), São Francisco-Estoril (84,8%) e Vila Toninho (59,1%). Between the mosquitoes specie tested to ISV infection the Ae. aegypti is the most positive  86,3%, follow Culex sp.: 53,5% and Ae. albopictus: 18,18%. Conclusion: The identification of the ISVs circulation in vectors mosquitoes to arbovirus are important tools to its control because the ISV can be directly involve at the transmission and the persistence arbovirus in the nature. More studies are necessary to identify which mechanisms were involved it. 

Palavras Chave

Insect-Specific vírus; Aedes aegypti; entomological surveillance