Dados do Trabalho

Título

Anellovirus species in serum of acute febrile patients from Mato Grosso, Central Western Brazil, 2019.

Introdução

The Anelloviridae family comprises the most diverse group of viruses within human virome comprising 155 species distributed across 30 genera, based on species demarcation criterion of a threshold of 69% nucleotide pairwise sequence identity of ORF-1, while genera are defined based on 44% nucleotide pairwise identity of the same region. Viral species described in humans are classified into four genera: the Alphatorquevirus, the Betatorquevirus, the Gammatorquevirus and the Hetorquevirus.

Objetivo (s)

To characterize seven Alphatorquevirus, two Betatorquevirus and five Gammatorquevirus genomic sequences found in the serum of febrile patients from Mato Grosso State, Central-Western Brazil, during 2019, using a metagenomic approach.

Material e Métodos

In total, 92 serum samples from patients showing symptoms between one to five days of disease onset, attending to basic health care units from 22 municipalities of Mato Grosso from January to December 2019 were included into 10 pools, subjected to viral nucleic acid extraction, library synthesis and NextSeq 500 sequencing. Phylogenetic relationships were inferred by Maximum likelihood (ML) method using coding ORF-1 aa sequences.

Resultados e Conclusão

Only three sequences belong to previously recognized anellovirus species: PP316247 is a Betatorquevirus homini15, PP316249 is an Alphatoquevirus homin15, and PP316252 is an Alphatoquevirus homin13, sharing, respectively, 92.05%, 95.61%, and 78.51% nt identity with the reference species. The remaining 11 viral genomes represent putative novel anelloviruses: PP316250, PP316251 and PP316255, share nt identity of 58.19%, 60.33% and 38.41%, respectively, closely related to Alphatoquevirus homin 7, 10 and 24 species. PP316253 shared 40.10% nt identitity with Alphatorquevirus homin13, and PP316254 shares 35.07% with the Alphatoquevirus homin15. The PP316248 sequence shares 62.54% of nt identity with Betatorquevirus homini19, PP316244 fits in a well-supported clade, closer to Gammatorquevirus homidi11, PP316243 fits in the unclassified Gammatorquevirus clade, closer to Gammatorquevirus homidi9. Gammatorquevirus homidi13 forms a well-supported monophyletic clade with two unclassified Gammatorquevirus clades containing PP316245 and PP316242. PP316246 allocates within an unclassified Gammatorquevirus clade closer to Gammatorquevirus homidi15. This study expands our understanding of AV diversity through the description of putative novel species and provides a broader range of complete genomic information for further field research.

Palavras Chave

Anelloviridae; diversidade viral; filogenia viral