Dados do Trabalho

Título

Evaluation of the antibacterial and antibiofilm activity of silver nanoparticles against resistant clinical isolates of Staphylococcus epidermidis

Introdução

Healthcare-Associated Infections (HAIs) are a growing problem and are responsible for increased public health spending, especially when associated with resistant, biofilm-producing bacteria, such as Staphylococcus epidermidis. S. epidermidis is the most common cause of associated infections associated with implants, cardiac pacemakers, and prosthetic valve endocarditis, in addition to being an excellent opportunistic biofilm former. Infections caused by resistant S. epidermidis and biofilm products are difficult to treat and can lead to serious complications, including sepsis, septic shock and infective endocarditis, constituting a serious public health problem. In this sense, the development of therapeutic approaches using nanostructures, such as silver nanoparticles (AgNPs), is promising. 

Objetivo (s)

Thus, this work aimed to evaluate the antibacterial and antibiofilm activity of AgNP and silver nitrate (AgNO3) in order to understand their potential action against resistant clinical isolates of Staphylococcus epidermidis. 

Material e Métodos

The determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNO3 and AgNP-C250 against C233, C266, C271 and C281 strains was performed by the broth microdilution method according to the Clinical and Laboratory Standards Institute. The determination of the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) of AgNO3 and AgNP against C233, C266, C271 and C281 strains was performed by the crystal violet method.

Resultados e Conclusão

The MIC and MBC of AgNO3 varied between 25 and 50 ug/m, while that of AgNP-C250 varied between 6.25 and 50 ug/mL and 25 and 50 ug/mL, respectively. The MBIC and MBEC of AgNO3 ranged between 12.5 to 50ug/mL, and 25 to 50 ug/mL, respectively. For AgNP-C250, MBIC was observed to vary between 1.56 and 12.50ug/mL, and MBEC to range from 25 to 50ug/mL. The results demonstrate greater effectiveness of AgNPs-C250 in relation to AgNO3 against S. epidermidis strains. In this way, AgNPs-C250 demonstrated the antibacterial and antibiofilm potential of these nanostructures, consolidating itself as a promising alternative for combating HAIs caused by resistant and biofilm-producing S. epidermidis.

Palavras Chave

bacterial resistance; Gram-positive bacteria; nanostructures; silver.